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ActiTaq exo Genotyping Master

5x concentrated

For further processing only.

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Order information
ActiTaq exo Genotyping Master material number and pack size:
Material Number Pack Size
06362281001 50 mL
Will be supplied as "ActiTaq delta exo Genotyping Master". Unit of measure is "mL".
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Overview
Hot start DNA master mix containing a 5'-3' exonuclease deficient hot start Taq DNA polymerase for optimal detection of mismatches.
The 5x concentrated master mix contains a chemically modified truncated Taq DNA Polymerase and all reagents (except primers, probes, and template) needed for real-time DNA detection assays with various probe formats except for hydrolysis probes due to the lack of 5'-3' exonuclease activity.
  • Optimize your SNP analysis.
    Discriminate paired and unpaired primer ends using an enzyme optimized for allele-specific PCR.
  • Simplify PCR set-up.
    Pipett and handle the hot start reaction mix at ambient temperature.
  • Simplify assay design.
    Create capable PCR assays with a minimum of optimization efforts, also multiplex PCR applications.
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Application
Use ActiTaq exo DNA Polymerase for:
  • SNP analysis and genotyping
  • Allele-specific PCR
  • Multiplexing up to 350 bp
  • Random primed PCR
 
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Specification
The master mix can be stored in the refrigerator (+2 to +8°C) for at least 4 week without loss of activity and performance. The complete PCR mix (Master + primers + probes + template) is stable for up to 24 hours at +15 to +25°C.
Appearance: Clear colorless solution
Performance test in qPCR (amplification with ApoB fragment): Corresponds to specification
Sensitivity (≤100 copies; negative control ≥40 cycles): Corresponds to specification
Stability: At -15 to -25°C within specification range for 24 months.
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License disclaimer

2:The purchase price of this product includes a limited, nontransferable license under US patent claims and corresponding patent claims outside the United States, licensed from Idaho Technology Inc., to use only this amount of the product for HybProbe assays and related processes described in said patents solely for the research and development activities of the purchaser. Diagnostic uses require a separate license from Roche.