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Collagenase Activity Test

Kit for determination of the enzymatic activity of Collagenases I and II

For use in quality control / manufacturing process only.

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Order information
Collagenase Activity Test material number and pack size:
Material Number Pack Size
08074461001 96 reactions
Unit of measure is "piece".
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Overview
Test kit for determination of collagenase activity for in-process control.
The Collagenase Activity Test kit is based on the chromogenic substrate FALGPA, which is a labeled tetrapeptide resembling the primary structure of collagen, and which is specifically hydrolyzed by all known collagenases but not by other proteases. This test offers a better precision and more convenience compared to other existing tests, and it is perfectly suited for process development and in-process control.
  • Reliability
    Accurate and reproducible results due to included calibration standards and high lot-to-lot consistency of the kit.
  • Convenience
    Solutions of FALGPA substrate and Collagenase standards are ready-to-use; no manual preparation of solutions or extraction of dyes needed.
  • High sensitivity
    Limit of detection: 0.32 FALGPA Units/mL.
  • Fast turnaround time
    Time-to-result: 30 minutes.
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Application
The kit is designed for determination of collagenase activity in in-process control.
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Specification
Measuring range: 0.32 - 1.15 FALGPA Units/mL
Precision: ≤15% (typically <10%)
Specificity: Collagenases I and II from Clostridium histolyticum
Assay time: 30 min approximately
Test format: 96-well plate, absorption at 340 nm
Stability: At -15 to -25°C within specification range for 12 months.