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Liberase T-Flex, Research Grade

0.2 μm filtered, lyophilizate

For life science research only. Not for use in diagnostic procedures.

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Order information
Liberase T-Flex, Research Grade material number and pack size:
Material Number Pack Size
05989132001 1 kit 1 x 500 mg Collagenase blend
2 x 15 mg Thermolysin
Will be supplied as "Liberase T-Flex Research Grade". Unit of measure is "piece".
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Overview
The Liberase T-Flex, Research Grade kit enables individual blends of collagenase and thermolysin in low level ratios. The enzymes are manufactured for applications in research where high purity, consistent quality and low levels of bacterial endotoxins are required for reproducible high cell yield and viability.
The Liberase T-Flex Research Grade kit contains highly purified collagenase class I and class II from Clostridium histolyticum. The two collagenase isoforms are blended in a precise ratio with each other. Highly purified thermolysin from Bacillus thermoproteolyticus is provided in a separate vial to allow individual adjustments of enzyme ratios for optimization of your tissue dissociation protocol.
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Application
Use Liberase T-Flex, Research Grade, for in vitro enzymatic dissociation of a broad range of tissues, enabling isolation of single cells. In particular intended for use in isolation procedures of pancreatic islets from several animal types.
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Specification
I. Collagenase I/II MPB
Appearance:
White lyophilized cake
Protein mass/bottle (target: 537 mg protein): 430-644 mg protein
Collagenase I content(target: 322 mg): 258-386 mg
Collagenase II content(target: 215 mg): 172-258 mg
Collagenase Ib, c part of Collagenase I: ≤10 area%
HPLC purity(total peak areas Collagenase I+II): ≥85 area%
Activity (Wünsch), calculated: 2,172-3,617 U/bottle
Ratio Collagenase II/total Collagenase: 0.3-0.5
Endotoxins: ≤10 EU/mg protein
Bioburden: ≤10 CFU/bottle

II. Thermolysin MPB
Appearance: White lyophilized cake
Protein mass/bottle (target: 15.0 mg protein): 12.0-18.0 mg protein
Activity target neutral protease (calculated): 130,500-234,000 U/bottle
HPLC purity (total peak area thermolysin): ≥85 area%
Endotoxins: ≤50 EU/mg protein
Bioburden: ≤10 CFU/bottle
Stability: At -15 to -25°C within specification range for 48 months.