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NADH, Grade II

for potassium test, disodium salt

For further processing only.

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Order information
NADH, Grade II material number and pack size:
Material Number Pack Size
11333925103 custom fill
Will be supplied as "NADH Di-Na, Grade II for Potassium Test". Unit of measure is "kg".
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Overview
NADH quality for enzymatic potassium test.
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Application
Use this special NADH as a cofactor in a diagnostic test for potassium, together with glutamate dehydrogenase to remove ammonia from the reaction mixture.
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Specification
Formula: C21H27N7O14P2Na2
Molecular weight: 709.4 D (NADH: 665.4 D)
Appearance: White to slightly yellowish amorphous powder
Solubility: Clear, colorless to slightly yellowish solution in water (c=50 mg/mL)
NADH-Na2 (from content found enzymatically, based on dry weight): ≥98%
NADH (enzymatic): ≥82%
NADH (A340, ε=6.3 [L x mmol-1 x cm-1]): ≥84%
NADH (A260, ε=14.3 [L x mmol-1 x cm-1]): ≥85%
Na (flame photometric): 6.5±0.5%
K (AAS): ≤250 ppm
Water (K. Fischer): ≤6%
NAD (enzymatic): ≤1%
AMP (enzymatic): ≤0.2%
Ethanol (GC): ≤4%
Reaction rates (Lactate dehydrogenase), based on standard:
Freshly dissolved sample: 95-105%
After 8 days incubation at +45°C: ≥95%
HPLC:
Freshly dissolved sample: ≥96.0 area%
After 8 days incubation at +45°C: ≥75 area%
A260/A340: ≤2.4
A260/A240: 1.57-2.17
Stability: At +2 to +8°C within specification range for 12 months.