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6-Phosphogluconolactonase

from Leuconostoc mesenteroides, lyophilizate

For further processing only.

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Order information
6-Phosphogluconolactonase material number and pack size:
Material Number Pack Size
11373129103 custom fill
Will be supplied as "6-Phosphogluconolactonase". Unit of measure is "kU".
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Overview
Hydrolase that catalyzes the conversion of 6-phosphogluconolactone to 6-phosphogluconate.
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Application
Use 6-Phosphogluconolactonase in diagnostic tests for the determination of creatine kinase or glucose in the combination with Hexokinase, Catalog Nos. 11 119 796 103, Glucose-6-phosphate Dehydrogenase, Catalog Nos. 10 186 783 103, 11 389 343 103, 11 293 206 103 or 10 190 454 103, and Phosphogluconate Dehydrogenase, Catalog No. 11 126 482 103.
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Specification
Nomenclature: 6-phosphogluconolactonase
Molecular weight: 38 kD (SDS)
Isoelectric point: 6.0
Michaelis constants (MES buffer, pH 6.5; +25°C):
6-Phosphogluconalactone: <1 x 10-7 mol/L
Inhibitors: (NH4)2SO4 (> 20 mmol/L), Mg2+ (>10 mmol/l), NaCl (>10 mmol/L). The enzyme is not inhibited by Cu2+, Zn2+, EDTA, 5.5'-dithiobis-2-nitrobenzoic acid, octanol (0.01%), Triton X-100 (1%) and Thesit (1%).
pH optimum: 6.0-7.5 (see figure)
Temperature dependence: See figure
pH stability: 7.0-9.0 (at +4°C, see figure)
Thermal stability: Up to +50°C (see figure)
Stability at different ionic strength: See figure
Specificity: 6-Phosphogluconolactone 100%, gluconolactone 0.5%
Appearance: White lyophilizate
Activity (+25°C, 6-phosphogluconolactone): ≥50 U/mg lyophilizate
Contaminants (expressed as percentage of 6-Phosphogluconolactonase activity):
Creatine kinase: ≤0.001
G6P-DH (with NAD): ≤0.02
Myokinase: ≤0.001
''NADPH oxidase'': ≤0.001
6- Phosphogluconate dehydrogenase (with NAD): ≤0.01
Function testing (with G6P-DH, reaction time up to 5 minutes): ≥98%
Signal stability (A340, 30 minutes): ≤0.02
Stability: At -15 to -25°C within specification range for 12 months.
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Performance
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