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Cholesterol Oxidase

from Brevibacterium species, expressed in E.coli, lyophilizate

For further processing only.

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Order information
Cholesterol Oxidase material number and pack size:
Material Number Pack Size
11479709103 custom fill
Will be supplied as "ChOD, Brevibacterium rec.". Unit of measure is "MU".
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Overview
Oxidoreductase that catalyzes the interconversion of cholesterol to cholest-4-en-3-one.
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Application
Use Cholesterol Oxidase in diagnostic tests for the determination of cholesterol in combination with Cholesterol Esterase, Catalog Nos. 10 129 046 103, 10 262 609 103, 11 015 923 103, 11 520 857 103 or 11 641 735 103.
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Specification
Nomenclature: Cholesterol:oxygen oxidoreductase
Molecular weight: 60 kD (native and SDS)
Isoelectric point: ~5.0
Michaelis constant (Phosphate buffer, 0.5 mol/l, pH 7.5; +25°C):
Cholesterol: 1 x 10-4 mol/L
Inhibitors: Hg2+, ZnCl2, SDS
Activators: Non ionic detergents
pH optimum: 5.5-8.0 (see figure)
Temperature dependence: See figure
pH stability: 5.0-10.0 (see figure)
Thermal stability: Up to +55°C (see figure)
Storage and Stability: No decrease in activity over 6 weeks at +35°C (see figure)
Specificity:
cholesterol 100%
pregnenolon 52%
stigmasterol 17%
dehydroandrosterone 0.5%
androsterone 0%
estradiol 0%
cholate 0%
Appearance: Yellow lyophilizate
Solubility: Clear, yellowish solution in water (c=10 mg/mL)
pH value: 6.0-7.0
Activity (+25°C, cholesterol): 10-20 U/mg lyophilizate
Protein (Biuret): 10-30 mg/100 mg lyophilizate
Contaminants (expressed as percentage of Cholesterol Oxidase activity):
Catalase: ≤6.0
Glucose oxidase: ≤0.01
"NADH oxidase": ≤0.01
Uricase: ≤0.01
Stability: At -15 to -25°C within specification range for 12 months. Store dry.
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Performance
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