Endoproteinase Lys-C, Sequencing Grade
from Lysobacter enzymogenes, solution
For further processing only.
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Endoproteinase Lys-C, Sequencing Grade material number and pack size: Material Number Pack Size 11051199103 custom fill
- Endoproteinase Lys-C, Sequencing Grade, can be used for specific cleavage of peptides. Endoproteinase Lys-C is a widely used serine protease, that specifically hydrolyzes amide, ester, and peptide bonds at the carboxylic side of lysin. Endoproteinase Lys-C is isolated from Lysobacter enzymogenes and is supplied as a solution.
Molecular weight: 33 kD (reduced), 30 kD (nonreduced)
pH optimum: 8.5-8.8
Inhibitors: DFP, TLCK, aprotinin, and leupeptin Appearance: Clear, colorless solution
Activity (+25°C, Chromozym® PL): No limit (U/mL)
Specific activity: ≥200 U/mg protein
Protein (A280): ≥0.1 mg/mL
Purity (SDS PAGE, homogeneity, Phast System®, 8-25% reducing terms): ≥90%
Specifity (HPLC, melittin, after 1 hour incubation): ≥90%
Specifity (HPLC, melittin, after 18 hours incubation): ≤5%
Stability: At -15 to -25°C within specification range for 12 months.