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Alanine Aminotransferase (ALT) (GPT)

from pig heart, suspension

For further processing only.

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Order information
Alanine Aminotransferase (ALT) (GPT) material number and pack size:
Material Number Pack Size
10153443103 custom fill
Will be supplied as "GPT from Pig Heart". Unit of measure is "kU".
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Application
Use Alanine Aminotransferase for designing your calibrator/control reagent and for the synthesis of unnatural L-amino acids from α-keto acids.
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Specification
Appearance: Slightly yellow suspension in ammonium sulfate, 3.2 mol/L
pH value: 5.5-6.5
Specific activity (+25 °C; L-alanine, α-ketoglutarate): ≥80 U/mg protein
Protein (Biuret): ≥10 mg/mL (standardized to 10±1 mg/mL)
Ammonium sulfate: 3.2±0.2 mol/L
Contaminants (expressed as percentage of Alanine Aminotransferase activity):
Glutamate dehydrogenase: ≤0.01
Aspartate aminotransferase (AST/GOT) : ≤0.03
Lactate dehydrogenase: ≤0.01
Malate dehydrogenase: ≤0.01
SVD free: Corresponds to specification
pH 5.5 treatment (30 minutes): Corresponds to specification
Stability: At +2 to +8°C within specification range for 12 months.