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Carboxypeptidase B, recombinant

from rat pancreas, expressed in Pichia pastoris, solution

For further processing only.

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Order information
Carboxypeptidase B, recombinant material number and pack size:
Material Number Pack Size
03358682103 30 kU
Will be supplied as "CpB rec.". Unit of measure is "MU".
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Overview
Carboxypeptidase B, recombinant, is intended to use in highly regulated production processes at pharmaceutical companies.
Carboxypeptidase B is a widely used metalloprotease, typically isolated from pancreas of different animals, that specifically releases arginine and lysine from the C-terminus of peptides and proteins. Roche has chemically synthesized a gene encoding for the amino acid sequence of the rat Carboxypeptidase B and has transformed the gene into the expression host Pichia pastoris, which expresses the recombinant Carboxypeptidase B as active protease with identical properties compared to the native Carboxypeptidase B.The production process is validated resulting in a very high lot-to-lot consistency.
  • Minimize the risk of virus contamination and the risk of animalrelated cross-contamination.
  • Rely on high purity. Minimize the risk of host cell protein contamination in your final product.
  • Increase the safety of your production processes with robust and reproducible performance and high lot-to-lot consistency.
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Application
Use the animal component-free and DIN EN ISO 13485-manufactured Carboxypeptidase B, recombinant, as critical raw material for the production of active pharmaceutical ingredients (API), i.e., insulin.
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Specification
Molecular weight: 34.6 kD
The product is manufactured according to DIN EN ISO 13485 .
No animal-derived products are used in the fermentation, purification and final formulation.
Appearance: Clear, colorless to slightly yellowish solution
Storage buffer: Tris/HCl, 33 mmol/L; ZnCl2, 0.1 mmol/L
pH (at +25°C): 7.5-8.5
Activity (with hippurylarginine): ≥400 U/mL
Total activity: 30 kU ±15%
Specific activity: ≥210 U/mg
Protein: 2.8±0.5 mg/mL
Purity (RP-HPLC): ≥85% according to master lot
Bioburden: ≤50 CFU/mL
Trypsin (with chromozym): ≤0.005%
Shipping conditions: Dry ice
Stability: At -15 to -25°C within specification range for 12 months.