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Endoproteinase Lys-C

from Lysobacter enzymogenes, lyophilizate

For further processing only.

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Order information
Endoproteinase Lys-C material number and pack size:
Material Number Pack Size
10476978103 3 U
Will be supplied as ''Endoproteinase Lys-C, Lysobacter enzymog.''. Unit of measure is ''U''.
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Overview
Endoproteinase Lys-C can be used for specific cleavage of peptides.
Endoproteinase Lys-C is a widely used serine protease, that specifically hydrolyzes amide, ester, and peptide bonds at the carboxylic side of lysin. Endoproteinase Lys-C is isolated from Lysobacter enzymogenes and is supplied as a lyophilizate.
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Application
Use Endoproteinase Lys-C for protein structure analysis and for sequence analysis. It is suitable to digest proteins in solution, in polyacrylamide gels or on blotting membranes.
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Specification
Molecular weight: 33 kD (reduced), 30 kD (nonreduced)
pH optimum: 8.5-8.8
Inhibitors: DFP, TLCK, aprotinin, and leupeptin
Appearance: White lyophilizate
Activity: ≥3 U/bottle
Specific activity: ≥150 U/mg protein
Unspecific cleavage peptides (HPLC, after 6 hours incubation): ≤5 area%
Stability: At +2 to +8°C within specification range for 12 months.