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NAD(P)H Dehydrogenase (quinone) (Diaphorase)

from pig heart, suspension

For further processing only.

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Order information
NAD(P)H Dehydrogenase (quinone) (Diaphorase) material number and pack size:
Material Number Pack Size
10153427103 custom fill
Will be supplied as "Diaphorase, Grade I from Pig Heart". Unit of measure is "g".
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Overview
Dehydrogenase that catalyzes the oxidation of dihydrolipoyl groups and has diaphorase activity.
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Application
Use the diaphorase activity of NAD(P)H Dehydrogenase (quinone) for the determination of NAD(P)H and many dehydrogenases when coupled with various dyes which act as hydrogen acceptors from NAD(P)H, e.g. tetrazolium salts.
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Specification
Appearance: Yellow suspension in ammonium sulfate, 3.2 mol/L
pH value: 5.5-6.5
Specific activity (+25°C, lipoate): ≥25 U/mg protein
Protein (Biuret): 10±1 mg/mL
Ammonium sulfate: 3.2±0.2 mol/L
SVD free: Corresponds to specification
pH 5.5 treatment (30 minutes): Corresponds to specification
Stability: At +2 to +8°C within specification range for 12 months.