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Alcohol Dehydrogenase, chemically modified

from yeast, lyophilizate

For further processing only.

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Order information
Alcohol Dehydrogenase, chemically modified material number and pack size:
Material Number Pack Size
11644980103 custom fill
Will be supplied as "Alcohol Dehydrogenase, Yeast, Modified". Unit of measure is "MU".
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Overview
Dehydrogenase that catalyzes the interconversion of alcohols to the corresponding aldehydes.
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Application
Use Alcohol Dehydrogenase in diagnostic tests for the determination of alcohol or aldehyde (formate).
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Specification
Nomenclature: Alcohol:NAD+ oxidoreductase
Molecular weight: 141 kD (pH 7.0)
Isoelectric point: 5.4-5.8
Michaelis constants (Phosphate buffer, pH 7.15, +25°C):
Ethanol: 1.3 x 10-2 mol/L
NAD: 7.4 x 10-5 mol/L
Acetaldehyde: 7.8 x 10-4 mol/L
NADH: 1.1 x 10-5 mol/L
Inhibitor constants (Phosphate buffer, pH 7.15, +25°C):
Ethanol: 4.3 x 10-2 mol/L
NAD: 6.1 x 10-4 mol/L
Acetaldehyde: 6.7 x 10-4 mol/L
NADH: 1.8 x 10-5 mol/L
Inhibitors:
-SH-reagents and heavy metals, such as derivatives, 4-chloromercuribenzoate, iodoacetic acid, N-substituted maleinimides, Hg2+, Ag+ and Cu2+.
-Complexing agents, e.g., o- phenanthroline, EDTA, oxalate.
-NAD analogs and NAD partial structures, e.g., NADP, NADH, ADP, ADP-ribose.
-Substances, which react with enzyme bound NAD, e.g., sulfite, hydroxylamine, cyanide.
-Substrate analogs, e.g., fluoroethanol.
-Oxidizers, e.g., H2O2 and aerial oxygen inactivate by oxidation of essential groups.
pH optimum: 9.0 (see figure for product Cat. No. 11 452 541 103)
Temperature dependence: See figure for product Cat. No. 11 452 541 103
pH stability: 6.0-8.0 (see figure for product Cat. No. 11 452 541 103)
Thermal stability: Up to +50°C (see figure for product Cat. No. 11 452 541 103)
Specificity: Alcohol dehydrogenase oxidizes primary alcohols. Isopropanol and secondary butanol are slowly oxidized, while higher secondary and tertiary alcohols do not react. Numerous aldehydes are reduced in the reverse reaction. The enzyme does not react with NADP.
Appearance: White lyophilizate
Solubility: Clear, colorless solution in water (c = 50 mg/mL)
pH value: 6.5-8.0
Activity (+25°C, ethanol): ≥25 U/mg lyophilizate
Contaminants (expressed as percentage of Alcohol Dehydrogenase activity):
Lactate dehydrogenase: ≤0.01
Malate dehydrogenase:≤0.01
Stability: At +2 to +8°C within specification range for 12 months.