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D-Lactate Dehydrogenase (D-LDH), Grade II

from Lactobacillus delbrückii, lyophilizate

For further processing only.

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Order information
D-Lactate Dehydrogenase (D-LDH), Grade II material number and pack size:
Material Number Pack Size
10679666103 custom fill
Will be supplied as ''D(-)-Lactate Dehydrogenase (D-LDH)''. Unit of measure is ''MU''.
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Overview
Dehydrogenase that catalyzes the interconversion of D(-)-lactate to pyruvate.
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Application
Use D-Lactate Dehydrogenase (D-LDH), Grade II, in a variety of diagnostic tests, e.g., in the determination of alanine aminotransferases, lactate or pyruvate. Used for the removal of pyruvate in determinations working with NADH (i.e., triglycerides, lipase, aldolase, aspartate aminotransferases, glutamate dehydrogenase).
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Specification
Nomenclature: D-lactate:NAD+ oxidoreductase
Michaelis constants (Tris maleate buffer, pH 8.0, +25°C):
D-lactate: 0.7 x 10-1 mol/L (NAD, 2 mmol/L)
Pyruvate: 1.2 x 10-3 mol/L (NADH, 0.1 mmol/L)
NADH: 7.1 x 10-5 mol/L (pyruvate, 20 mmol/L)
pH optimum: 7.0
pH stability: 4.0-10.0
Thermal stability: Up to +50°C
Specificity: Lactate dehydrogenase is specific for D(-)-lactate, L(+)-lactate does not react.
Appearance: White to yellowish lyophilizate
Solubility: Clear, colorless to slightly yellowish solution in water (c=10 mg/mL)
pH value (c=10 mg/mL in water): 5.7-6.7
Activity (+25°C, pyruvate): ≥150 U/mg lyophilizate
Specific activity: ≥300 U/mg protein
Protein (Biuret): No limit, approximately 0.4-0.7 mg/mg lyophilizate
Contaminants (expressed as percentage of D-Lactate Dehydrogenase activity):
Alcohol dehydrogenase: ≤0.01
GIucose dehydrogenase: ≤0.01
Malate dehydrogenase: ≤0.1
Succinate dehydrogenase: ≤0.01
Stability: At +2 to +8°C within specification range for 12 months. Store dry.