• Broad spectrum

    of products for

    your industry

Glycerol Kinase (GK)

from Bacillus stearothermophilus, solution

For further processing only.

expand | collapse
+-
Order information
Glycerol Kinase (GK) material number and pack size:
Material Number Pack Size
10691666103 custom fill
Will be supplied as "Glycerokinase from Bac.stearothermophil.". Unit of measure is "kU".
+-
Overview
Enzyme that catalyzes the phosphorylation of glycerol to glycerol-3-phosphate.
+-
Application
Use Glycerol Kinase for diagnostic tests for the determination of triglycerides together with Glycerol-3-phosphate Oxidase, Catalog Nos. 11 582 003 103 and 11 654 730 103, and Lipoprotein Lipase, Catalog No. 11 145 991 103.
+-
Specification
Nomenclature: ATP:glycerol 3-phosphotransferase
Molecular weight: 230 000 D (Sephadex G 200), 4 x 58 000 D (SDS-gel electrophoresis)
Michaelis constants (Glycine buffer, pH 9.8; +30°C):
Glycerol: 4.4 x 10-5 mol/L
Inhibitors: Unknown; Inhibitors of glycerokinase from Candida mycoderma do not inhibit the glycerokinase from Bacillus stearothermophilus.
pH optimum: 10.0-10.5 (see figure for product Cat. No. 11 499 530 103)
Temperature dependence: See figure for product Cat. No. 11 499 530 103
pH stability: 5.0-11.0 (see figure for product Cat. No. 11 499 530 103)
Thermal stability: Up to +60° C (see figure for product Cat. No. 11 499 530 103)
Appearance: Clear, colorless to slightly yellowish solution in Tris buffer; pH approximately 7.3, stabilized, potential particles as result of recristallized salts
Activity (+25°C, glycerol): ≥500 U/mL
Specific activity: ≥85 U/mg protein
Protein (Biuret): No limit
Contaminants (expressed as percentage of Glycerol Kinase activity):
Hexokinase: ≤0.01
"NADH oxidase": ≤0.005
Stability: At +2 to +8°C within specification range for 12 months.