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L-Lactate Dehydrogenase (L-LDH)

from pig muscle, for use of AST/GOT-Determination according to IFCC recommendations, lyophilizate

For further processing only.

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Order information
L-Lactate Dehydrogenase (L-LDH) material number and pack size:
Material Number Pack Size
10254754103 custom fill
Will be supplied as "LDH IFCC-quality from Hog Muscle". Unit of measure is "MU".
Additional formulation: Suspension in glycerol solution, Catalog No. 10 417 718 103
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Overview
Dehydrogenase that catalyzes the interconversion of specific for L(+)-lactate to pyruvate.
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Application
Use L-Lactate Dehydrogenase in a variety of diagnostic tests for the removal of pyruvate in determinations working with NADH (i.e., triglycerides, lipase, aldolase, aminotransferases, glutamate dehydrogenase).
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Specification
Nomenclature: L-lactate:NAD+ oxidoreductase
Molecular weight: 140 kD
Isoelectric point: 4.6
Michaelis constants (Phosphate buffer, pH 7.5; +25°C):
Pyruvate: 1.5 x 10-4 mol/L (NADH: 0.18 mmol/L)
L-lactate: 3.3 x 10-3 mol/L (NAD: 0.5 mmol/L)
NADH: 1.1 x 10-5 mol/L (Pyruvate: 0.6 mmol/L)
NAD: 6.7 x 10-5 mol/L (L-lactate: 34 mmol/L)
Inhibitors: Oxamate, pyruvate (excess), oxalate, Ag+, Hg2+, Cu2+
pH optimum: 3.0-7.0 (see figure)
Temperature dependence: See figure
pH stability: 5.5-8.5 (see figure)
Thermal stability: Up to +40°C (see figure)
Specificity: Lactate dehydrogenase is specific for L(+)-lactate, D(-)-lactate does not react. Glyoxylate is also a lactate dehydrogenase substrate. Apart from pyruvate some 2-oxoacids are reduced. NAD analogs (e.g., APAD) react at similar rates.
Appearance: White lyophilizate
Solubility: Clear colorless solution in water (c=10 mg/mL)
pH value (c=10 mg/mL in water): 6.0-7.0
Activity (+30°C, pyruvate; according to IFCC recommendations):
for Aspartate aminotransferase (AST/GOT) determination: ≥50 U/mg lyophilizate
for Alanine aminotransferase (ALT/GPT) determination: ≥50 U/mg lyophilizate
Activity (mean value of both determinations): ≥50 U/mg lyophilizate
Contaminants (expressed as percentage of Lactate Dehydrogenase activity, assayed according to the IFCC recommendations):
Aspartate aminotransferase (AST/GOT): ≤0.001
unspecificity of Lactate dehydrogenase: ≤0.005
Alanine aminotransferase (ALT/GPT): ≤0.001
unspecificity of Lacatae dehydrogenase: ≤0.005
SVD free: Corresponds to specification
pH 5.5 treatment (30 minutes): Corresponds to specification
Stability: At +2 to +8°C within specification range for 12 months.
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Performance
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