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Glucose Oxidase (GOD), Grade I

from Aspergillus niger overproducer, lyophilizate

For further processing only.

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Order information
Glucose Oxidase (GOD), Grade I material number and pack size:
Material Number Pack Size
12158566103 custom fill
Will be supplied as "GOD, RG I, rec., Lyo.". Unit of measure is "MU".
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Overview
Oxidoreductase that catalyzes the conversion of D-glucose to D-glucono-1,5-lactone which hydrolyzes spontanously to gluconate.
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Application
Use Glucose Oxidase (GOD), Grade I for the determination of α-amylase and D-glucose or O2.
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Specification
Nomenclature: β-D-glucose:oxygen 1-oxidoreductase
Molecular weight: 79 kD
Isoelectric point: 4.3
Michaelis constants (Glucose):
Acetate buffer, pH 5.0, +25°C: 3.6 x 10-2 mol/L
Potassium phosphate buffer, 0.2 mol/L, pH 7.5, +25°C: 4.8 x 10-2 mol/L
Inhibitors: Ag+, Hg2+, Cu2+, 4-choloromercuribenzoate, D-arabinose (50%). FAD binding is inhibited by several nucleotides.
pH optimum: 7.0 (see figure)
Temperature dependence: See figure
pH stability: See figure
Thermal stability: See figure
Specificity: Glucose oxidase is specific for β-D-glucose. O2 can be replaced by hydrogen acceptors such as 2,6-dichlorophenol indophenol.
Appearance: Yellowish lyophilizate
Conductivity (1%, w/v): ≤250 μS/cm
Activity (+25°C, glucose): ≥300 U/mg lyophilizate
Contaminants (expressed as percentage of Glucose Oxidase activity):
Amylase: ≤0.01
Catalase: ≤0.5
Saccharase: ≤0.01
Stability: At +2 to +8°C within specification range for 24 months. Store dry.
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Performance
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