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Glucose Oxidase (GOD), chemically modified

from Aspergillus niger overproducer, lyophilizate

For further processing only.

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Order information
Glucose Oxidase (GOD), chemically modified material number and pack size:
Material Number Pack Size
11485938103 custom fill
Will be supplied as "GOD, Asp.niger, Bound to Dextran". Unit of measure is "MU".
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Overview
Oxidoreductase that catalyzes the conversion of D-glucose to D-glucono-1,5-lactone which hydrolyzes spontanously to gluconate.
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Application
Use Glucose Oxidase (GOD), chemically modified for the determination of α-amylase and D-glucose or O2. The chemical modification generates an increased liquid stability of the enzyme.
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Specification
Nomenclature: β-D-glucose:oxygen 1-oxidoreductase
Molecular weight: 79 kD
Isoelectric point: 4.3
Michaelis constants (Glucose):
Acetate buffer, pH 5.0, +25°C: 3.6 x 10-2 mol/L
Potassium phosphate buffer, 0.2 mol/L, pH 7.5, +25°C: 4.8 x 10-2 mol/L
Inhibitors: Ag+, Hg2+, Cu2+, 4-choloromercuribenzoate, D-arabinose (50%). FAD binding is inhibited by several nucleotides.
pH optimum: 7.0 (see figure for product Cat. No. 12 158 566 103)
Temperature dependence: See figure for product Cat. No. 12 158 566 103
pH stability: See figure for product Cat. No. 12 158 566 103
Thermal stability: See figure for product Cat. No. 12 158 566 103
Specificity: Glucose oxidase is specific for β-D-glucose. O2 can be replaced by hydrogen acceptors such as 2,6-dichlorophenol indophenol.
Remark: The modified enzyme is especially suited for liquid stable applications with extended shelf life requirements.
Appearance: Yellowish white lyophilizate
pH value (c=40 mg/mL in water): 6.5-7.5
Activity (+25°C, glucose): ≥20 U/mg lyophilizate
Contaminants (expressed as percentage of Glucose Oxidase activity):
Catalase: ≤20 U/mg lyophilizate
Stability: At +2 to +8°C within specification range for 12 months. Store dry.