Hexokinase (HK), chemically modified
from yeast overproducer, lyophilizate
For further processing only.
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Hexokinase (HK), chemically modified material number and pack size: Material Number Pack Size 11370600103 custom fill
- Recombinant enzyme that converts hexose to hexose-6-phosphate.
- Use Hexokinase in diagnostic tests for blood glucose using the hexokinase method and for the determination of creatine kinase. The chemical modification generates an increased liquid stability of the enzyme.
Nomenclature: ATP:D-hexose 6-phosphotransferase
Molecular weight: 57 kD (SDS-PAGE)
Isoelectric point: 4.5-5.0
Michaelis constants (D-glucose):
Phosphate buffer, 0.1 mol/L, pH 7.0; +25°C: 3.05 x 10-4 mol/L
Phosphate buffer, 0.1 mol/L, pH 7.4; +30°C: 1.90 x 10-4 mol/L
Tea buffer, 0.1 mol/L, pH 7.6; +25°C: 2.30 x 10-4 mol/L
Michaelis constants (ATP):
Tris buffer, 0.1 mol/L, pH 7.6; +28°C: 1.60 x 10-4 mol/L
Tea buffer, 0.1 mol/L, pH 7.6; +25°C: 1.90 x 10-4 mol/L
Inhibitors: EDTA, SH-blocking compounds, sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxymethylglucose, lyxose.
Activators: Mg2+, catecholamines
pH optimum: 7.0-10.0 (see figure for product Cat. No. 11 119 796 103)
Temperature dependence: See figure for product Cat. No. 11 119 796 103
pH stability: 5.0-9.0 (see figure for product Cat. No. 11 119 796 103)
Thermal stability: See figure for product Cat. No. 11 119 796 103
Specificity: Hexokinase phosphorylates D-glucose, D-fructose, D-mannose, D-glucosamin, 2-deoxyglucose. L-Arabinose, D-xylose, L-rhamnose, D-galactose, D-lactose, sucrose, maltose, trehalose, raffinose, N-acetyl glucosamine do not react. ATP can be partially replaced by other nucleotides. Appearance: White lyophilizate
Solubility: Clear, colorless solution in water (c=40 mg/mL)
pH value (c=40 mg/mL in water): 6.5-7.5
Activity (+25°C, glucose): ≥40 U/mg lyophilizate
Contaminants (expressed as percentage of hexokinase activity):
Alcohol dehydrogenase: ≤0.001
Creatine kinase: ≤0.001
Glutamate dehydrogenase: ≤0.05
Glutathione reductase: ≤0.005
''NADH oxidase'': ≤0.001
6-Phosphogluconate dehydrogenase: ≤0.001
Phophoglucose isomerase: ≤0.002
Glucose: ≤0.3 μg/mg lyophilizate
Stability: At +2 to +8°C within specification range for 18 months.
Remark: This enzyme is especially suited for liquid stable applications with extended shelf life requirements.